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Updated: Aug 11, 2026

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
[A boy with cholera from India]
A M van Furth1, R D Croughs, L Terpstra
1VU Medisch Centrum, Postbus 7057, 1007 MB Amsterdam. am.vfurth@vumc.nl
Insights
A child contracted cholera after drinking contaminated water during international travel. Prompt medical intervention with intravenous rehydration led to a full recovery within three days.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Public Health
Background:
- Cholera is a severe diarrheal disease caused by Vibrio cholerae.
- Imported cases of cholera are rare in non-endemic regions like the Netherlands.
Observation:
- A 7-year-old boy traveling from India presented with severe watery diarrhea and loss of consciousness.
- Rice water-like stools and comma-shaped, motile, Gram-negative rods were observed.
- Vibrio cholerae O1 El Tor, serotype Inaba, was identified in fecal cultures.
Findings:
- The patient recovered within three days following intravenous rehydration.
- The infection was linked to consuming bottled water from New Delhi.
- Cholera can be fatal due to severe dehydration.
Implications:
- This case highlights the risk of imported infectious diseases during international travel.
- Prompt diagnosis and treatment are crucial for managing cholera effectively.
- Standard hospital admission protocols are sufficient as cholera is not person-to-person transmissible.
Abstract:
A 7-year-old Indian boy travelling from India to the United Kingdom was brought to the Emergency Clinic of Airport Medical Services at Schiphol airport in Amsterdam, the Netherlands. He had had watery diarrhoea in the aircraft and had lost consciousness. In view of the strong indications for cholera and the rice water-like diarrhoea, he was admitted to the paediatric ward of the VU Medical Centre where intravenous rehydration was carried out. He recovered within three days. A large number of comma-shaped, motile, Gram-negative rods were found in the faeces. After two days, the faeces culture revealed Vibrio cholerae O1 El Tor, serotype Inaba. On the day of the flight, the patient had drunk a litre of water from a bottle that later turned out to have been from the New Delhi water supply. Cholera is rare as an import disease in the Netherlands. Due to the severe dehydration, the infection can run a serious course and even be fatal. The infection is not transmitted from person to person. Therefore, no special measures are needed when a patient with cholera is admitted to hospital.
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