[Screening of phytase-producing strain and its optimal solid state phytase-producing conditions]

Area of Science:

• Enzymology and Microbial Fermentation
• Agricultural Science

Context:

• Phytate in animal feed reduces nutrient availability and contributes to environmental pollution.
• Phytase enzymes hydrolyze phytate into accessible inositol and phosphoric acid.
• Optimizing phytase production is key to enhancing animal nutrition and reducing ecological impact.

Purpose:

• To isolate and mutate a phytase-producing strain, Trichoderma Viride LH374.
• To determine the optimal conditions for solid-state fermentation (SSF) of this strain for maximum phytase production.
• To quantify the phytase yield under optimized SSF conditions.

Summary:

• A novel phytase-producing strain, Trichoderma Viride LH374, was developed through isolation and mutation.
• Optimal solid-state fermentation conditions were identified as a straw:rice bran ratio of 8:2, initial pH 6.5, 30°C, 96 hours, and 60% moisture content.
• Under these conditions, the strain achieved a significant phytase accumulation of 1,580 U/g.

Impact:

• The optimized SSF process significantly enhances phytase production by Trichoderma Viride LH374.
• Increased phytase availability can lower animal feed costs and improve nutrient utilization.
• This research contributes to more sustainable animal agriculture by mitigating phytic acid's negative effects.