Related Experiment Video
Updated: Jul 21, 2026

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
Published on: February 18, 2014
Equilibrium dialysis data and the relationships between preferential interaction parameters for biological systems in
1Department of Chemistry, 111 Willard Hall, Kansas State University, Manhattan, Kansas 66506-3701, USA. pesmith@ksu.edu
Abstract:
Equilibrium dialysis data has provided valuable information concerning the preferential interaction of a cosolvent with a biomolecule in aqueous solutions. Here, we formulate the experimental data in terms of Kirkwood-Buff (KB) theory, resulting in equations that provide a simple physical picture of the dialysis experiment and thereby the interaction of a cosolvent with a biomolecule. These results are then used to establish exact relationships between preferential interaction coefficients, defined in different ensembles and/or using different concentration scales, in terms of KB integrals. It is then argued that the molality based equilibrium dialysis data represent the situation most relevant to computer simulations performed in either open or closed systems.
Related Concept Videos
The Equilibrium Constant
Calculating the Equilibrium Constant
For example, gaseous nitrogen dioxide forms dinitrogen tetroxide according to this equation:
The Equilibrium Binding Constant and Binding Strength
The Equilibrium Binding Constant and Binding Strength
Extraction: Partition and Distribution Coefficients
For extracting a solute from an aqueous phase into an organic...
Dialysis

