Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Recombination in the human IGK locus.

Anton W Langerak1, Jacques J M van Dongen

  • 1Erasmus MC, Department of Immunology, Rotterdam, The Netherlands. a.langerak@erasmusmc.nl

Critical Reviews in Immunology
|February 14, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ABO: A 3D stroma-supported culture platform enabling full human B-lymphopoiesis for disease modeling and gene therapy development.

Cell reports. Medicine·2026
Same author

Diagnosis of primary CNS lymphoma through molecular and immune biomarkers in cerebrospinal fluid (PRICELUS): a multicentre, prospective, cohort study.

The Lancet. Haematology·2026
Same author

Challenges and future directions of AIRR-seq-based diagnostics.

Immunoinformatics (Amsterdam, Netherlands)·2026
Same author

Flow Cytometric Assessment of Pertactin- and Tetanus Toxoid-Specific B-Cell Kinetics After Tdap Booster Vaccination in Healthy Adults.

Vaccines·2026
Same author

Familial patterns of immune dysregulation in CVID: insights from B- and T-cell phenotyping and antibody profiling.

Frontiers in immunology·2026
Same author

Reply.

The Journal of allergy and clinical immunology·2026

The human immunoglobulin kappa (IGK) locus exhibits complex V(D)J recombination, involving various DNA elements and regulatory factors like E2A. Aberrant IGK recombinations, while infrequent, hold diagnostic value for B-cell malignancies.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • The human immunoglobulin kappa (IGK) locus is highly complex due to numerous elements involved in V(D)J recombination.
  • Various classical and non-classical recombinations, including pseudohybrid joints, occur in normal and malignant human B cells.

Purpose of the Study:

  • To explore the intricate mechanisms of V(D)J recombination at the human IGK locus.
  • To investigate the role of regulatory factors, such as E2A, in IGK recombination.
  • To understand the diagnostic and clinical significance of IGK recombinations.

Main Methods:

  • Analysis of V(D)J recombination events in human B cells (normal and malignant).
  • Investigation of DNA methylation, histone acetylation, and nuclear positioning in IGK regulation.

Related Experiment Videos

  • Assessment of the role of the E2A transcription factor in IGK recombination induction.

    • Main Results:

    • Observed various types of recombinations, including signal joints and pseudohybrid joints, in human B cells.
    • Identified E2A as a key factor in inducing human endogenous IGK recombination.
    • Found that aberrant IGK recombinations leading to chromosomal translocations are rare compared to the IGH locus.

    Conclusions:

    • The human IGK locus undergoes ordered and regulated V(D)J recombination, influenced by epigenetic modifications and nuclear organization.
    • E2A plays a crucial role in IGK recombination during B-cell development.
    • IGK recombinations are valuable targets for PCR-based clonality and minimal residual disease (MRD) studies in B-cell malignancies.