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Eph/Ephrin membrane proteins: a mammalian expression vector pTIg-BOS-Fc allowing rapid protein purification

Bryan W Day1, Fiona M Smith, Ke Chen

  • 1Leukaemia Foundation of Queensland Laboratory, Queensland Institute of Medical Research. P.O Royal Brisbane Hospital, Queensland 4029, Australia. Bryan.Day@qimr.edu.au

Protein and Peptide Letters
|February 14, 2006
PubMed

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...