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Expression and purification of active WW domains of FBP11/HYPA and FBP28/CA150
Yusuke Kato1, Yoriko Sawano, Masaru Tanokura
1Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. amtanok@mail.ecc.u-tokyo.ac.jp.
Protein and Peptide Letters
|February 14, 2006
Abstract:
Production of GST-fused WW domains of FBP proteins was increased using the bubbling cultivation method for E. coli. Purified WW domains of FBP11 and FBP28 bound a PL motif peptide with dissociation constants (K(D)) of 248 +/- 27 and 1880 +/- 280 microM, respectively.
