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Updated: Aug 4, 2026
09:58
RhoC GTPase Activation Assay
Published on: August 22, 2010
GEF and glucosylation assays on liposome-bound Rac
1CNRS-Institut de Pharmacologie, Moleculaire et Cellulaire, Valbonne, France.
Methods in Enzymology
|February 14, 2006
Abstract:
Rac binds tightly to lipid membranes through a lipid modification. The influence of the lipid membrane environment on the multiple interactions of Rac has not been well documented. In this chapter, we detail a method to prepare geranyl-geranylated Rac bound to liposomes of defined composition. With this method, one can dissect some lipid-protein interactions that facilitate the interaction of Rac with other proteins such as guanine nucleotide exchange factors and bacterial toxins.
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