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Updated: Aug 11, 2026

Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy
Published on: July 20, 2022
Use of TIRF microscopy to visualize actin and microtubules in migrating cells
1Institut Curie--CNRS, Paris, France.
Abstract:
Total internal reflection fluorescence (TIRF) is the technique of choice to visualize and quantify cellular events localized at the basal plasma membrane of adherent cells. By selectively illuminating the first 200 nm above the basal membrane, it allows maximal resolution in the vertical z-axis. In this chapter, I describe a prism-based TIRF setup and the procedures to visualize the actin and microtubule cytoskeleton in migrating astrocytes. TIRF microscopy provides quantitative information on the organization of the cytoskeleton in both fixed and live migrating cells.
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