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Peak compression in reversed-phase gradient elution.

Uwe D Neue1, Daniel H Marchand, Lloyd R Snyder

  • 1Waters Corporation, Milford, MA 01757, USA. Uwe_Neue@Waters.com

Journal of Chromatography. A
|February 14, 2006
PubMed
Summary
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Gradient compression in linear gradient elution is a valid concept, reconciling experimental results with theoretical predictions. Ignoring this phenomenon previously led to discrepancies between observed and expected peak widths in chromatography.

Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Discrepancies exist between theoretical predictions and experimental results for peak widths in linear gradient elution.
  • The role of gradient compression in chromatography has been poorly understood or incorrectly dismissed in previous reports.

Purpose of the Study:

  • To investigate the phenomenon of gradient compression in linear gradient elution.
  • To clarify the reasons for the historical differences between experimental observations and theoretical models in chromatography.

Main Methods:

  • An experimental program was designed and executed.
  • Data from experiments were compared with theoretical models.

Main Results:

  • Peak widths in linear gradient elution were observed to be larger than theoretical predictions when gradient compression was ignored.

Related Experiment Videos

  • Experimental data aligned with theory when gradient compression was properly accounted for.

    • Conclusions:

    • The concept of gradient compression is validated as a correct and important phenomenon in chromatography.
    • Gradient compression plays a crucial role in accurately predicting and understanding peak widths in linear gradient elution.