Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

A stability-indicating HPLC assay method for docetaxel.

B Mallikarjuna Rao¹, Arpita Chakraborty, M K Srinivasu

¹Analytical Research, Custom Pharmaceutical Services, Dr. Reddy's Laboratories, Hyderabad 500049, India. bmrao67@rediffmail.com

Journal of Pharmaceutical and Biomedical Analysis

|February 14, 2006

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

F-minimum latency, F-maximum latency and F-estimate as early markers of neuropathy in type 2 diabetes mellitus: a cross-sectional study.

BMJ neurology open·2026

Same author

Chasing clarity in acute pancreatitis: Comparing computed tomography severity index and APACHE-II score in predicting severity in Indian patients.

Medical journal, Armed Forces India·2026

Same author

Synergistic microbial consortia in the bioremediation of heavy metal-contaminated wastewater: mechanisms and sustainability perspectives.

Environmental geochemistry and health·2026

Same author

Oxygen-depleted and ferruginous seawater composition imprinted in Early Cretaceous Fe-rich Al-glauconites in marginal marine deposits.

Scientific reports·2025

Same author

Enhancing diagnostic accuracy in carpal tunnel syndrome: integrating the median-ulnar F-wave latency differentials with symptom severity score and katz hand diagram.

Journal of family medicine and primary care·2025

Same author

Cortical Excitability and Heart Rate Variability in Response to Short Duration Transauricular Vagus Nerve Stimulation in Healthy Adults.

Neuromodulation : journal of the International Neuromodulation Society·2025

A new stability-indicating HPLC method accurately quantifies docetaxel and its impurity DCT-1. This validated assay is crucial for assessing docetaxel stability and purity in pharmaceutical analysis.

Area of Science:

Pharmaceutical Analysis
Analytical Chemistry
Chromatography

Background:

Docetaxel is a vital chemotherapeutic agent.
Assessing drug stability and purity is critical for patient safety.
Existing analytical methods may not adequately detect docetaxel degradation products.

Purpose of the Study:

To develop and validate a novel stability-indicating HPLC method for docetaxel.
To accurately quantify docetaxel in the presence of its degradation products.
To establish a reliable method for impurity profiling of docetaxel.

Main Methods:

Development of a gradient high-performance liquid chromatography (HPLC) method.
Utilized a Hichrom RPB HPLC column for separation.
Employed a mobile phase of water and acetonitrile.

Related Experiment Videos

Detection at 230 nm with a flow rate of 1.0 ml/min.

Main Results:

Successfully separated docetaxel from its degradation products.
Quantitatively estimated docetaxel and the impurity DCT-1.
The developed gradient LC method demonstrated high performance for stability indicating analysis.

Conclusions:

The novel HPLC method is stability-indicating and suitable for docetaxel analysis.
This method provides accurate quantification of docetaxel and its key impurity.
The validated method supports quality control and stability studies of docetaxel formulations.