Related Experiment Video
Updated: Aug 11, 2026

Purification of Pathogen Vacuoles from Legionella-infected Phagocytes
Published on: June 19, 2012
Analysis of Rab1 recruitment to vacuoles containing Legionella pneumophila
Abstract:
Legionella pneumophila is an intracellular bacterial pathogen that utilizes a type IV secretion system to subvert the function of the Rab1 GTPase. Bacterial proteins translocated into host cells mediate the accumulation of the Rab1 protein on vacuoles containing Legionella pneumophila. Assays used to investigate recruitment of Rab1 by L. pneumophila are described. These assays can be used to determine host and bacterial factors required for L. pneumophila subversion of the host secretory pathway.
More Related Videos
08:34Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages
Published on: February 22, 2017
09:12Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
Published on: March 10, 2020
Related Concept Videos
Rab Proteins
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab Cascades
Intralumenal Vesicles and Multivesicular Bodies