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Updated: Aug 11, 2026
08:51
The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
Published on: February 12, 2022
Fluorescent microscopy-based assays to study the role of Rab22a in clathrin-independent endocytosis
Methods in Enzymology
|February 14, 2006
Abstract:
The endocytic and trafficking route followed by proteins that enter cells independently of clathrin is not well understood. In HeLa cells, a distinct endocytic and recycling pathway is followed for such clathrin-independent cargo proteins, and we have characterized the role of Rab proteins in this process. Here we describe cell-based flourescence assays to examine the effects of expression and depletion of Rab22 on endosomal morphology and endocytic recycling.
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