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Purification and functional analysis of a Rab27 effector munc 13-4 using a semi-intact platelet dense-granule
Methods in Enzymology
|February 14, 2006
Abstract:
We have demonstrated that small GTPase Rab27 regulates dense-granule secretion in platelets. Using Rab27a affinity chromatography, we purified Munc 13-4 as a novel Rab27a interacting protein from platelet cytosol. This chapter describes the purification of Munc 13-4 and an in vitro assay system analyzing the mechanism of dense-granule secretion in platelets. The activity of Munc 13-4 is tested in this assay.
