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Updated: Aug 11, 2026

Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
Published on: January 30, 2020
Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces
Gholam Khodakaramian1, Sarah Lissenden, Bertolt Gust
1Institute of Genetics, University of Nottingham Nottingham NG7 2UH, UK.
Abstract:
We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage phiC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.
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