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[Effects of N-linked oligosaccharide chains on expression of integrin beta1 and apoptosis in glomerular mesangial

Wen-li Cai1, Song-min Huang, Li Zeng

  • 1Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.

Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition
|February 16, 2006
PubMed
Abstract

Insights

N-linked oligosaccharide chains (TM) inhibit N-glycosylation, decreasing beta1 integrin and cyclin D1 expression in glomerular mesangial cells. This inhibitor also increases cell apoptosis and reduces proliferation, offering potential for proliferative nephropathy treatment.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Nephrology

Background:

  • N-glycosylation is crucial for protein function.
  • Aberrant glycosylation is implicated in kidney diseases.
  • Glomerular mesangial cells (GMCs) play a role in kidney function and disease.

Purpose of the Study:

  • To investigate the effects of TM, an N-linked oligosaccharide inhibitor, on GMCs.
  • To explore TM's role in cell apoptosis, proliferation, and beta1 integrin/cyclin D1 expression.
  • To identify potential therapeutic strategies for proliferative nephropathy.

Main Methods:

  • Cultured rat GMCs were treated with varying doses of TM.
  • Flow cytometry assessed beta1 integrin expression and apoptosis rates.
  • Immunohistochemistry measured cyclin D1 expression, and MTT assays evaluated cell proliferation.

Main Results:

  • TM significantly decreased beta1 integrin and cyclin D1 expression.
  • TM treatment led to increased GMC apoptosis and reduced proliferation.
  • All observed effects of TM were dose-dependent.

Conclusions:

  • TM suppresses beta1 integrin expression by inhibiting glycoprotein glycosylation, impacting cell adhesion.
  • TM increases apoptosis and decreases proliferation and cyclin D1 expression in GMCs.
  • These findings suggest TM as a potential agent for treating proliferative nephropathy.

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