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Updated: Aug 11, 2026
Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection
Published on: June 23, 2023
Expression of Nm23 in gliomas and its effect on migration and invasion in vitro
Shin Jung1, Yun-Woong Paek, Kyung-Sub Moon
1Department of Neurosurgery, Chonnam National University Hospital and Medical School, Gwangiu, South Korea. sjung@chonnam.ac.kr
Background:
Despite the well-documented importance of Nm23 in the control of metastasis, there is currently a paucity of data regarding the role of this gene family in the control of glioma invasion.
Materials And Methods:
Nm23-H1 expression in gliomas was assessed via immunohistochemistry, Western blot, RT-PCR and Northern blot analyses. The migration and invasion ability were also investigated in primary glioma culture cells, human glioma cell lines and nm23-H1 transfectant, using an in vitro brain slice invasion model and a simple scratch technique.
Results:
Although no significant correlations were detected between nm23-H1 expression and pathological grade, the endogenous nm23-H1 expression in gliomas was found to be inversely correlated with their migratory abilities. Additionally, the nm23-H1 transfectant resulted in a reduction of approximately 45% of the migratory ability and suppressed the invasiveness of the parental cell line.
Conclusion:
Our overall findings suggest that nm23-H1 may play an important role in the suppression of glioma invasion and migration.
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