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Related Experiment Videos

Heparin accelerates gelsolin amyloidogenesis.

Ji Young Suk1, Fuming Zhang, William E Balch

  • 1Department of Chemistry and The Skaggs Institute of Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

Biochemistry
|February 16, 2006
PubMed
Summary
This summary is machine-generated.

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Heparin accelerates gelsolin amyloid formation by promoting beta-sheet structure. Its sulfated structure and molecular weight influence aggregation, suggesting extracellular matrix glycosaminoglycans impact tissue-selective deposition in gelsolin amyloidoses.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Extracellular Matrix Biology

Background:

  • Gelsolin amyloid disease involves tissue-selective deposition of amyloid fragments.
  • Heparin is known to accelerate gelsolin amyloidogenesis.
  • Proteases generate amyloidogenic fragments from gelsolin variants.

Purpose of the Study:

  • To identify structural features of heparin that promote amyloid formation in disease-associated gelsolin fragments.
  • To elucidate the mechanism by which heparin accelerates gelsolin amyloidogenesis.
  • To investigate the role of heparin's structural properties in fibril formation and aggregation.

Main Methods:

  • Kinetic studies to monitor amyloid formation.
  • Electron microscopy for structural analysis.

Related Experiment Videos

  • Use of heparin analogues to probe structure-activity relationships.
  • Dynamic light scattering coupled with size exclusion chromatography to analyze aggregates.

    • Main Results:

    • Heparin efficiently accelerates gelsolin amyloid formation by enabling intermolecular beta-sheet formation.
    • Sulfation of heparin is crucial for accelerating amyloidogenesis, with acceleration proportional to molecular weight.
    • Heparin promotes the formation of soluble aggregates and templates fibril formation.
    • Heparin's sulfated structure affords solubility to aggregating peptides.

    Conclusions:

    • Heparin's structural features, particularly sulfation and molecular weight, are key drivers of gelsolin amyloidogenesis.
    • Heparin acts as a template for fibril formation and influences aggregate solubility.
    • The findings suggest that extracellular glycosaminoglycans contribute to the tissue-selective deposition observed in gelsolin amyloidoses.