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Related Experiment Videos

Analysis of electrotonic coupling in patterned neuronal networks.

L Lauer1, A K Vogt, C Kauff

  • 1Max Planck Institute for Polymer Research, Mainz, Germany.

IEE Proceedings. Nanobiotechnology
|February 16, 2006
PubMed
Summary

Researchers guided neuronal network formation using laminin-patterned substrates. They discovered functional and electrotonic synapses, and derived a model to determine neurite radius and its correlation with pattern width.

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Area of Science:

  • Neuroscience
  • Biomaterials Science
  • Cell Biology

Background:

  • Laminin-patterned substrates guide neuronal cell migration and neurite outgrowth.
  • Culturing brain stem slices on these substrates allows for the formation of grid-shaped neuronal networks.

Purpose of the Study:

  • To investigate neuronal network formation on laminin-patterned substrates.
  • To assess interconnections between neurons in these artificial networks.
  • To develop an electrical model for electrotonically coupled cells.

Main Methods:

  • Microcontact printing of laminin on substrates.
  • Culturing ultrathin brain stem slices (E15-E18) from Sprague-Dawley rats.
  • Electrophysiological assessment using double patch-clamp recordings.

Related Experiment Videos

  • Optical assessment via microinjection of fluorescent dyes.
  • Electrical modeling using PSpice simulations.

    • Main Results:

    • Both functional and electrotonic synapses were detected between neurons.
    • An electrical model for electrotonically coupled cells was derived, representing neurites as cylindrical cables and gap junctions as ohmic resistors.
    • The average inner radius of neurites was determined to be approximately 0.1 microm.
    • A correlation was found between the path-width of the pattern and the diameter of neurites.

    Conclusions:

    • Laminin-patterned substrates effectively guide neuronal network formation.
    • The derived electrical model provides insights into electrotonic coupling in neurites.
    • Neurite growth is influenced by the geometry of the patterned substrate.