First look at the effect of overexpression of TIGR/MYOC on the transcriptome of the human trabecular meshwork
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Updated: Aug 11, 2026
Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
Published on: June 3, 2018
Teresa Borrás1, Paul A Bryant, Sarah S Chisolm
1Department of Ophthalmology, School of Medicine, University of North Carolina, at Chapel Hill, 6109 Neuroscience Research Building CB 7041, 103 Mason Farm Road, Chapel Hill, NC 27599-7041, USA. tborras@med.unc.edu
Abstract:
Wild-type TIGR/MYOC is a secreted protein implicated in the development of steroid glaucoma. Mutations in TIGR/MYOC have been linked to some patients who develop elevated intraocular pressure (IOP) and glaucoma. Because there is evidence of some other factors contributing to the TIGR/MYOC causative role in glaucoma, and because substantial increased levels of a particular cellular mRNA and protein might alter expression of other host genes, we began to investigate the effect of TIGR/MYOC overexpression on the transcriptome of human trabecular meshwork cells. We used a recombinant adenovirus carrying wild-type TIGR/MYOC cDNA, primary HTM cells, 300 viral particles per cell and U133 Affymetrix GeneChips. Our results indicate that 2361 out of the 22,284 genes (10.6%) were altered more than two-fold (p
