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Passage of red blood cells through the sinusoidal wall of the spleen
Abstract:
The migration of blood cells across the wall of splenic sinusoids has been studied by means of serial sections in rats inoculated with murine erythroblastosis virus (MEV). The sinusoidal walls of the normal spleen have no permanent openings. In the normal spleen, mature red blood cells and blood cells in the latest stages of maturation can be seen in diapedesis. The predominant form of transmural migration is intercellular. In MEV-inoculated animals, in which there is a vigorous erythroblastic proliferation, large numbers of erythroblasts are in transmural passage. Serial sections show that these cells migrate through the cytoplasm of the endothelial lining cells. On the basis of these observations, it is suggested that the transcellular mode of migration may be related to the delivery of newly formed red blood cells into the circulation while the intercellular mode may be part of the mechanism by which the spleen controls the removal of red cells from the circulation.
Insights
Researchers studied blood cell migration in rat spleens using serial sections after murine erythroblastosis virus (MEV) inoculation. They observed transcellular and intercellular migration, suggesting roles in red blood cell delivery and removal.
Area of Science:
- Hematology
- Cell Biology
- Spleen Function
Background:
- Splenic sinusoidal walls lack permanent openings in normal conditions.
- Mature red blood cells and late-stage precursors exhibit diapedesis across the sinusoidal wall.
- Intercellular transmural migration is the predominant mode in normal spleens.
Purpose of the Study:
- To investigate blood cell migration across splenic sinusoids.
- To examine the mechanisms of transmural passage in normal and virus-infected rats.
- To elucidate the roles of different migration modes in splenic function.
Main Methods:
- Utilized serial section microscopy.
- Studied Sprague Dawley rats inoculated with murine erythroblastosis virus (MEV).
- Analyzed cellular transmural passage through splenic sinusoidal endothelium.
Main Results:
- MEV inoculation induced vigorous erythroblastic proliferation and significant erythroblast transmural passage.
- Observed erythroblasts migrating through the cytoplasm of endothelial lining cells (transcellular migration).
- Confirmed intercellular migration as the predominant mode in normal spleens.
Conclusions:
- Transcellular migration by erythroblasts may facilitate the delivery of new red blood cells into circulation.
- Intercellular migration likely plays a role in the spleen's regulation of red blood cell removal.
- The spleen employs distinct cellular migration pathways for red blood cell homeostasis and turnover.