Relation between fluorescence decays and temporal evolution of excited states
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Updated: Aug 11, 2026

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
Published on: September 16, 2014
János Erostyák1, Géza Makkai, Andrea Buzády
1Department of Experimental Physics, Faculty of Sciences, University of Pécs, H-7624, Pécs, Ifjúság u. 6, Hungary. erostyak@fizika.ttk.pte.hu
Abstract:
A differential equation system describing the temporal evolution of excited substates and fluorescence emission were tested using a DOPRI algorithm. The numerical solutions show that there is significant difference in the measurable parameters according to the type of connectivity among the excited substates. In the globally connected case, the fluorescence emission exhibits a double exponential behavior, and the first moment of the emitted spectrum decays with stretched exponential characterized by beta < 1. In the diffusive case the fluorescence emission cannot be always fitted with double exponential, and the first moment of the emitted spectrum may decay with stretched exponential characterized by beta > 1. Details of modeling and the possibilities of drawing conclusions are also presented.
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