Genes expressed in a turrid venom duct: divergence and similarity to conotoxins

The toxoglossate mollusks are a large group of venomous animals (>10,000 species) conventionally divided into three groups, the cone snails, the auger snails, and the turrid snails; turrids account for >90% of the biodiversity of toxoglossans. Only the venoms of cone snails have been intensively investigated, with little work focused on turrids. We report the first broad characterization of genes expressed in venom ducts of any turrid species. Twenty-three different cDNA clones encoding putative toxins were characterized from the venom duct of the turrine species Lophiotoma olangoensis Olivera 2002 and belong to 16 different gene families. Of the 16 different Lophiotoma olangoensis gene families that encode putative toxins, for only 1 was there clear evidence of sequence similarity with any conotoxin gene family. The I-like gene family of Lophiotoma olangoensis was found to be related to the K channel-targeted I(2) conotoxin superfamily. Most putative Lophiotoma toxins are cysteine-rich polypeptides, with a significant fraction much larger (>80 amino acids) than the toxins from cone snails. A small number were not cysteine-rich but had hydrophobic amino acid clusters interspersed with arginine residues. This is only 1 of >10,000 different turrid venoms that needs to be characterized. From this study, a common origin with Conus for one family of putative turrid toxins is indicated.