Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chromatography with two mobile phases.

M Wang1, S Hou, J F Parcher

  • 1Chemistry Department, University of Mississippi, University, MS 38677, USA.

Analytical Chemistry
|February 16, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mass spectrometric inverse gas chromatography:  investigation of polymeric phase transitions.

Analytical chemistry·2011
Same author

Chromatography with dynamically created liquid "stationary" phases: methanol and carbon dioxide.

Analytical chemistry·2003
Same author

Unified chromatography with CO2-based binary mobile phases.

Analytical chemistry·2003
Same author

Gas-liquid chromatography with a volatile "stationary" liquid phase.

Analytical chemistry·2002
Same author

Tech.Sight. Blurring the boundaries.

Science (New York, N.Y.)·2001
Same author

Lattice-fluid model for gas-liquid chromatography.

Journal of chromatography. A·1999
Same journal

Machine Learning-Assisted Label-Free SERS Decoding of Mitochondrial Molecular Dynamics in Ovarian Granulosa Cells during Aging.

Analytical chemistry·2026
Same journal

Revealing the Regulatory Interplay of NHE1 mRNA and Na<sup>+</sup> in Cancer Cells Using a DNA Nanosensor.

Analytical chemistry·2026
Same journal

Towards Cellular Resolution of Tryptic Peptides in Tissue Sections by MALDI MS Imaging: A Focus on Enzyme Application and Reproducibility.

Analytical chemistry·2026
Same journal

Bioinspired Bilayer Hydrogel Colorimetric Sensor Array for Low-Temperature Food Freshness Analysis.

Analytical chemistry·2026
Same journal

Quartz Crystal Microbalance-Based Point-of-Care Testing Systems: Principles, Device Design, and Applications.

Analytical chemistry·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
See all related articles

This study explores chromatography using two mobile phases, methanol and carbon dioxide, creating a unique retention window. This method enhances solute separation by defining distinct lower and upper limits for retention times.

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Chromatography

Background:

  • Chromatographic methods are crucial for separating and analyzing chemical mixtures.
  • Conventional chromatography relies on a single mobile phase and a stationary phase.

Purpose of the Study:

  • To investigate the behavior of chromatographic columns utilizing two mobile phases (methanol and carbon dioxide).
  • To characterize the "retention window" created by this dual-phase system.
  • To determine the impact of varying experimental conditions on solute retention.

Main Methods:

  • Utilized a fused-silica-lined stainless steel capillary column.
  • Employed mixtures of methanol and carbon dioxide as the eluent under varying pressures and temperatures.
  • Calculated phase compositions and densities using the Peng-Robinson equation of state.

Related Experiment Videos

  • Determined mobile phase residence times via tracer pulse chromatography.
  • Measured partition coefficients of benzene and retention times of neon.

    • Main Results:

    • The eluent separated into distinct liquid (methanol-rich) and gas (CO2-rich) phases within the column.
    • Neon served as an accurate dead time marker, indicating no dissolution in the liquid phase.
    • A defined retention window was observed, with lower and upper bounds defined by gas and liquid phase residence times, respectively.
    • Benzene's partition coefficient varied with density and temperature, showing a maximum at intermediate temperatures.

    Conclusions:

    • Chromatography with two mobile phases creates a unique "retention window" for enhanced solute separation.
    • The absence of a stationary phase, combined with the dual mobile phases, defines the retention limits.
    • This approach offers a novel strategy for chromatographic analysis by controlling retention through mobile phase composition and conditions.