Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Method for internal standard introduction for quantitative analysis using on-line solid-phase extraction LC-MS/MS.

Yazen Alnouti¹, Ming Li, Olga Kavetskaia

¹Pharmacokinetics, Dynamics and Metabolism, Pfizer Global Research and Development, Groton, CT 06340, USA.

Analytical Chemistry

|February 16, 2006

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Drug-drug interaction assessments of clinical transporter inhibition: Learnings from the Transporter Working Group of the International Consortium for Innovation and Quality in Pharmaceutical Development.

Drug metabolism and disposition: the biological fate of chemicals·2026

Same author

Application of modelling of cell monolayer permeation data to generate input parameters compatible with in vitro-in vivo translation of blood-brain-barrier disposition of drugs.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2026

Same author

Chameleonic Efficiency Shapes Ligand Access and Binding at TRPA1's Membrane-Facing Site.

Journal of medicinal chemistry·2025

Same author

Total drug's last stand on phospholipidosis: Unbound drug rules!

Drug metabolism and disposition: the biological fate of chemicals·2025

Same author

Beyond single-dose human mass balance study: minimizing subject confinement and enabling true steady-state assessment with a novel multiple-dose design proposal.

Drug metabolism and disposition: the biological fate of chemicals·2025

Same author

Property-Based Prediction Uncovers Intestinal Excretion as an Elimination Route of Small-Molecule Drugs.

Journal of medicinal chemistry·2025

A new method for on-line internal standard (IS) introduction in LC-MS/MS analysis improves accuracy and precision. This technique allows direct plasma analysis without sample preparation, simplifying quantitative workflows.

Area of Science:

Analytical Chemistry
Biomolecular Analysis

Background:

Quantitative analysis using LC-MS/MS often requires precise internal standard (IS) introduction.
Traditional methods involve premixing analyte and IS, which can be time-consuming and prone to errors.

Purpose of the Study:

To develop a novel on-line method for introducing internal standards (IS) in quantitative LC-MS/MS analysis.
To enable direct plasma analysis without extensive sample preparation.

Main Methods:

Analyte and IS are introduced sequentially into the sample injection loop via an autosampler.
IS is delivered on-line from a microreservoir.
The method was validated using model compounds in rat plasma with on-line solid-phase extraction (SPE) LC-MS/MS.

Main Results:

Related Experiment Videos

Achieved injection accuracy and precision comparable to off-line IS introduction.
Maintained chromatographic parameters like elution time and peak width.
Demonstrated surrogate IS effectiveness in compensating for signal variations and suppression during on-line SPE LC-MS/MS.

Conclusions:

The developed on-line IS introduction method is reliable and accurate for quantitative LC-MS/MS.
This approach facilitates direct, nonvolumetric plasma analysis, eliminating the need for sample manipulation and preparation.
The methodology streamlines quantitative workflows by integrating sample preparation and analysis.