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Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
Published on: March 7, 2018
Kinase-catalyzed modification of gold nanoparticles: a new approach to colorimetric kinase activity screening
Zhenxin Wang1, Raphaël Lévy, David G Fernig
1Centre for Nanoscale Science, Department of Chemistry and School of Biological Sciences, The University of Liverpool, Liverpool L69 7ZD, U.K.
Journal of the American Chemical Society
|February 16, 2006
Abstract:
Peptide-stabilized gold nanoparticles have been enzymatically biotinylated by a kinase-catalyzed reaction using biotin-ATP as a cosubstrate. Upon mixing with avidin-modified particles, solutions of biotinylated particles change color from red to blue, indicating aggregation of particles. On the basis of this reaction, we have developed a simple colorimetric test to monitor kinase inhibitor activity.
