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Updated: Aug 11, 2026
Gene Trapping Using Gal4 in Zebrafish
Published on: September 29, 2013
Zebrafish transgenic Enhancer TRAP line database (ZETRAP)
Benjamin G H Choo1, Igor Kondrichin, Sergey Parinov
1Institute of Molecular and Cell Biology, 61 Biopolis Dr, Proteos, 138673, Singapore. benjaminchoogh@yahoo.com
Background:
The zebrafish, Danio rerio, is used as a model organism to study vertebrate genetics and development. An effective enhancer trap (ET) in zebrafish using the Tol2 transposon has been demonstrated. This approach could be used to study embryogenesis of a vertebrate species in real time and with high resolution.
Description:
The information gathered during the course of systematic investigation of many ET transgenic lines have been collected and compiled in the form of an online database--the Zebrafish Enhancer TRAP lines database (ZETRAP).
Conclusion:
ZETRAP is a web-based system that provides data and information to the scientific community about the developmental, genetic and genomic aspects of transgenic zebrafish lines obtained using Tol2 transposon-mediated transgenesis. The current version (version 1.0) contains description of 27 ET lines that express EGFP in various organs and tissues, for example, heart, brain, notochord, gut, etc. It also includes information on insertion sites of the Tol2 transposon in these lines.
