[Optical coherence tomography in the diagnosis of vocal folds]

Optical coherence tomography (OCT) is a new, non-invasive method for investigating biological tissue, with a beam penetration of up to 3 mm. In this study, OCT was used on porcine and human larynxes. The porcine vocal folds were assigned to defined areas and examined by OCT followed by traditional histomorphological analysis. We were able to validate this new method by demonstrating that both OCT and the histological assays showed a clear demarcation of the epithelium mucosae from the deeper layers. OCT application to human vocal folds delivered same results. The current standard procedure for ensuring an accurate diagnosis if tissue is malignant, is still an invasive one. Our results show that OCT allows the collection of crucial information on the quality of morphological changes on a vocal fold without the necessity of being invasive. Thus, we propose that OCT be introduced as a new method for the detection of morphological changes in the vocal fold complementary to other established methods.