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Updated: Aug 11, 2026

Curtain Flow Column: Optimization of Efficiency and Sensitivity
Published on: June 12, 2016
High-efficiency liquid chromatography on conventional columns and instrumentation by using temperature as a variable
François Lestremau1, Andrew Cooper, Roman Szucs
1Pfizer Analytical Research Centre-Ghent University, Krijgslaan 281 S4-bis, B-9000 Ghent, Belgium.
Abstract:
High plate numbers were obtained in conventional LC by coupling columns and by using temperature to reduce the viscosity of the mobile phase. At 80 degrees C up to eight columns of 25 cm x 4.6 mm I.D. packed with 5 microm ODS particles could be coupled generating 180,000 effective plates while the pressure drop was only 350bar. For routine work, a set of four columns is preferred. The analysis times on one column operated at 30 degrees C and 1 mL/min flow rate and on four columns at 80 degrees C and 2 mL/min flow rate are the same in isoeluotropic conditions while the resolution is doubled. Multicolumn systems were successfully applied in isocratic and gradient mode for the analysis of pharmaceutical and environmental samples.
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