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Plasma cell tumour progression in iMycEmu gene-insertion mice
1Laboratory of Genetics, Center for Cancer Research (CCR), National Cancer Institute (NCI), NIH, Bethesda, MD 20892, USA.
The Journal of Pathology
|February 17, 2006
Summary
Gene insertion of Myc(His) in mice predictably causes extraosseous plasmacytomas (PCTs), a B-cell neoplasm. This mouse model mimics human plasma cell neoplasms, aiding research into multiple myeloma progression.
Area of Science:
- Oncology
- Immunology
- Genetics
Background:
- Gene-targeted iMyc(Emu) mice with Myc(His) develop B-cell lineage neoplasms.
- Extraosseous plasmacytomas (PCTs) are a subset of these neoplasms requiring further investigation.
Purpose of the Study:
- To investigate the development and characteristics of PCTs in Myc(His)-expressing mice.
- To explore the role of Myc deregulation in PCT pathogenesis.
- To establish a mouse model for human plasma cell neoplasms.
Main Methods:
- Histological, immunohistochemical, and molecular genetic analyses.
- Investigation of tumor-bearing iMyc(Emu) mice on a mixed genetic background.
- Analysis of gene expression using cDNA macroarrays.
- Characterization of PCT-derived cell lines.
Main Results:
- 20.8% of tumor-bearing iMyc(Emu) mice developed PCTs by 500 days.
- PCTs overexpressed Myc(His), leading to B-cell neoplasia.
- A T(12;15) translocation was observed in one cell line, altering Myc deregulation.
- The mouse model recapitulates key aspects of human plasma cell neoplasms.
Conclusions:
- Gene insertion of Myc(His) reliably induces PCT development in mice.
- This mouse model provides a valuable system for studying plasma cell neoplasms, including multiple myeloma.
- Understanding Myc deregulation in PCTs offers insights into human cancer progression.
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