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Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
Published on: April 6, 2017
Aged garlic extract and its constituents inhibit platelet aggregation through multiple mechanisms
Gillian L Allison1, Gordon M Lowe, Khalid Rahman
1School of Biomolecular Sciences, Liverpool John Moores University, Liverpool L3 3AF, England, UK.
The Journal of Nutrition
|February 18, 2006
Summary
Aged garlic extract (AGE) inhibits platelet aggregation and reduces calcium levels in platelets, suggesting a synergistic mechanism for cardiovascular health. This study explores AGE
Area of Science:
- Cardiovascular Science
- Biochemistry
- Pharmacology
Background:
- Cardiovascular disease is a major health concern.
- Platelet aggregation plays a key role in cardiovascular events.
- Aged garlic extract (AGE) is known to reduce cardiovascular disease parameters, but its mechanisms are unclear.
Purpose of the Study:
- To investigate the mechanisms by which AGE inhibits platelet aggregation.
- To determine the effects of AGE constituents and amino acids on platelet function.
- To explore the role of intracellular calcium in AGE's anti-platelet activity.
Main Methods:
- Assessing the effect of AGE on platelet aggregation in a concentration-dependent manner.
- Testing individual AGE constituents and amino acids for anti-aggregatory and disaggregatory properties.
- Measuring intraplatelet calcium levels in response to AGE and platelet activators.
Main Results:
- AGE significantly inhibited platelet aggregation and displayed disaggregatory properties.
- Specific AGE constituents and amino acids showed anti-aggregatory effects, with synergistic inhibition observed when combined.
- AGE abolished the increase in intraplatelet calcium induced by A23187 or ADP.
Conclusions:
- AGE exerts anti-platelet effects through multiple synergistic biochemical pathways.
- The inhibition of intraplatelet calcium increase is a key mechanism for AGE's action.
- AGE holds potential for cardiovascular disease management through its anti-aggregatory properties.
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