Related Experiment Video
Updated: Aug 11, 2026

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
Published on: July 14, 2015
Preparation of a monolithic column for weak cation exchange chromatography and its application in the separation of
Yinmao Wei1, Xiaodong Huang, Ruixian Liu
1Institute of Modern Separation Science, Northwest University, Xi'an, China. ymwei@nwu.edu.cn
Abstract:
A procedure for the preparation of a monolithic column for weak cation exchange chromatography was presented. The structure of the monolithic column was evaluated by mercury intrusion. The hydrodynamic and chromatographic properties of the monolithic column--such as back pressures at different flow rates, effects of pH on protein retention, dynamic loading capacity, recovery, and stability--were determined under conditions typical for ion-exchange chromatography. The prepared monolithic column might be used in a relatively broad pH range from 4.0 to 12.0 and exhibited an excellent separation to five proteins at the flow rates of both 1.0 and 8.0 mL/min, respectively. In addition, the prepared column was first used in the purification and simultaneous renaturation of recombinant human interferon gamma (rhIFN-gamma) in the extract solution with 7.0 mol/L guanidine hydrochloride. The purity and specific bioactivity of the purified rhIFN-gamma in only one chromatographic step were obtained to be 93% and 7.8 x 10(7) IU/mg, respectively.
Related Concept Videos
Ion-Exchange Chromatography
Ion Exchange
Types Of Column Chromatography
Gel Filtration Chromatography
When the...
Principles Of Column Chromatography
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
