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[Superoxide production of polymorphonuclear leukocytes in endotoxin shock and antioxidative effect of coenzyme Q10]
1First Department of Surgery, Nippon Medical School, Tokyo, Japan.
Abstract:
The present study was designed to determine the superoxide production of polymorphonuclear leukocytes (PMN) in endotoxin shock and the antioxidative effect of coenzyme Q10. PMN were collected from rats before and after the intravenous administration of endotoxin. PMN were also collected from healthy humans. Superoxide production of PMN was measured by the cytochrome C method. Lipid peroxide in the liver was examined by the TBA method. Twelve hours after the intravenous administration of 5 mg/kg endotoxin, superoxide production of PMN was 13 times higher than that in the control rats, and the amount of lipid peroxide in the liver was increased. In coenzyme Q10 treated endotoxin shock rats, superoxide production was significantly decreased, and lipid peroxide production in the liver was also inhibited. These findings suggest that endotoxin has a priming effect on the superoxide production of PMN and induces lipid peroxidation. Furthermore coenzyme Q10 has an anti-endotoxin shock effect by inhibiting the superoxide production of PMN and lipid peroxidation in the liver.
Insights
Endotoxin shock significantly increases polymorphonuclear leukocyte (PMN) superoxide production and liver lipid peroxide. Coenzyme Q10 effectively mitigates these effects, offering an anti-endotoxin shock therapy.
Area of Science:
- Biochemistry
- Immunology
- Pharmacology
Context:
- Endotoxemia is a critical condition characterized by systemic inflammation.
- Polymorphonuclear leukocytes (PMN) play a key role in the inflammatory response.
- Oxidative stress, indicated by superoxide production and lipid peroxidation, is a hallmark of endotoxin shock.
Purpose:
- To investigate the impact of endotoxin administration on PMN superoxide production.
- To evaluate the role of lipid peroxide accumulation in the liver during endotoxin shock.
- To determine the potential therapeutic effect of coenzyme Q10 on these pathological processes.
Summary:
- Intravenous endotoxin administration in rats led to a 13-fold increase in PMN superoxide production and elevated liver lipid peroxide levels.
- Coenzyme Q10 treatment significantly reduced PMN superoxide production and inhibited hepatic lipid peroxidation in endotoxin-shocked rats.
- These results indicate that endotoxin primes PMN for enhanced superoxide release and induces oxidative damage.
Impact:
- Coenzyme Q10 demonstrates a protective effect against endotoxin shock by modulating key oxidative stress markers.
- Findings suggest coenzyme Q10 as a potential therapeutic agent for managing endotoxemia and its associated complications.
- This study elucidates the mechanisms underlying endotoxin-induced oxidative stress and the antioxidant capacity of coenzyme Q10.