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Related Experiment Videos

Changes in lipid environment decrease Na, K-ATPase activity in obstructive nephropathy.

N Brunskill1, C Hayes, J Morrissey

  • 1Department of Medicine, Washington University School of Medicine, St. Louis, Missouri.

Kidney International
|May 1, 1991
PubMed
Summary
This summary is machine-generated.

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Unilateral ureteral obstruction reduces sodium-potassium adenosine triphosphatase (Na,K-ATPase) activity in kidneys. This decrease is linked to altered lipid environment, not enzyme amount, and is reversible after obstruction relief.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Physiology

Background:

  • Unilateral ureteral obstruction (UUO) impairs kidney sodium and water reabsorption.
  • Reduced Na,K-ATPase activity in nephron segments is a suspected cause of these abnormalities.
  • The precise mechanisms behind decreased Na,K-ATPase activity during obstruction remain unclear.

Purpose of the Study:

  • To investigate the mechanisms responsible for reduced Na,K-ATPase activity in obstructed kidneys.
  • To determine if the decrease in Na,K-ATPase activity is due to enzyme quantity, latency, or alterations in the membrane environment.

Main Methods:

  • Preparation of basolateral membrane vesicles (BMVs) from obstructed and contralateral kidneys of rats with 24-hour UUO.
  • Assay of Na,K-ATPase activity and latency in BMVs.

Related Experiment Videos

  • Immunoblotting to quantify Na,K-ATPase alpha subunit.
  • Reconstitution of Na,K-ATPase activity by incubating BMVs with liposomes.

    • Main Results:

    • Na,K-ATPase activity was significantly reduced in BMVs from obstructed kidneys compared to controls.
    • No difference in enzyme latency or alpha subunit amount was observed between obstructed and control kidneys.
    • Lipid exchange with liposomes reconstituted Na,K-ATPase activity in BMVs from obstructed kidneys to near-normal levels.

    Conclusions:

    • Decreased Na,K-ATPase activity in UUO is primarily due to alterations in the lipid environment of the basolateral membrane, not reduced enzyme quantity or latency.
    • These functional changes are reversible upon release of the obstruction.
    • The findings highlight the importance of membrane lipid composition in regulating renal transporter function during obstruction.