Quantitative autoradiographic analysis of excitatory amino acid receptors in the cat spinal cord

Using quantitative autoradiography, we have studied the density and distribution of N-methyl-D-aspartate (NMDA), kainate and AMPA receptors and the binding site for the sodium-dependent EAA transporter in sections from the cat spinal cord. NMDA, kainate and AMPA receptors were found in highest concentrations in laminae I and II of the dorsal horn. Lower levels of all receptors were seen in other regions of the spinal cord grey matter. The distribution of the sodium-dependent transporter was unlike that of any of the receptor populations with highest levels found in the ventral horn with slightly lower levels in other regions of grey matter. The pattern of binding sites was consistent throughout all levels of the spinal cord.