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In-Vivo Calcium Imaging of Sensory Neurons in the Rat Trigeminal Ganglion
Published on: February 9, 2024
A local GABAergic system within rat trigeminal ganglion cells
H Hayasaki1, Y Sohma, K Kanbara
1Department of Anatomy, Osaka Medical College, Takatsuki, Osaka 569-8686, Japan.
The European Journal of Neuroscience
|February 21, 2006
Summary
This study reveals the presence and function of the GABAergic system in rat trigeminal ganglia (TG). GABA is expressed in neurons and satellite cells, modulating neuronal activity through GABA(A) receptors.
Area of Science:
- Neuroscience
- Molecular Biology
- Cell Biology
Background:
- The trigeminal ganglion (TG) is crucial for transmitting sensory information.
- The role of the GABAergic system in the TG is not fully understood.
- GABAergic signaling is vital for neuronal inhibition and modulation.
Purpose of the Study:
- To investigate the expression and function of the GABAergic system in the Sprague-Dawley rat TG.
- To identify GABA and GABA(A) receptor subunit expression in TG neurons and satellite cells.
- To explore the functional impact of GABA on TG neurons.
Main Methods:
- Reverse transcription-polymerase chain reaction (RT-PCR) for mRNA analysis.
- In situ hybridization and immunohistochemistry for protein localization.
- Whole-cell patch clamp electrophysiology to study GABA-induced currents.
- K+-induced GABA release experiments.
Main Results:
- GAD65/67 mRNAs and various GABA(A) receptor subunit mRNAs were detected in TG.
- GAD65 and GABA immunoreactivity were found in neuronal cell bodies, while satellite cells showed strong GABA positivity.
- GABA application induced Cl- currents in all examined TG neurons, indicating functional receptors.
- GABA release was observed from TG cells.
Conclusions:
- The GABAergic system is present and functional within the rat TG.
- GABA likely acts as a diffusible neurotransmitter, potentially modulating somatic inhibition in the TG.
- GABAergic signaling in the TG may play a significant role in sensory processing.

