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Updated: Aug 11, 2026

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
Redox cycling of adrenaline and adrenochrome catalysed by mitochondrial Complex I
Maria Luisa Genova1, Nagwa M Abd-Elsalam, El Sayed M E Mahdy
1Dipartimento di Biochimica "G. Moruzzi," Università di Bologna, Via Irnerio 48, 40126 Bologna, Italy. marialuisa.genova@unibo.it
Abstract:
Complex I in bovine heart submitochondrial particles catalyses the NADH-supported generation of superoxide anion; adrenaline is oxidised by superoxide to adrenochrome that, on its hand, is reduced by Complex I, thus establishing a redox cycle that amplifies the superoxide production. The routes in Complex I for superoxide formation and for adrenochrome reduction appear to be different, since they have a different sensitivity to Complex I inhibitors. The results are discussed in terms of current assays for superoxide detection and of pathologies linked to catecholamine oxidation.
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