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Genotoxic and aneugenic properties of an imidazole derivative

M A Carballo1, A S Hick, S Soloneski

  • 1Citogenética y Genética Toxicológica. Departamento de Bioquímica Clínica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina. macarballo2003@yahoo.com.ar

Journal of Applied Toxicology : JAT
|February 21, 2006
PubMed

Insights

Thiabendazole (TBZ) can induce genetic damage, increasing sister chromatid exchanges in hamster cells and affecting cell division in human lymphocytes. Further research is needed to fully understand its mutagenic and aneugenic potential.

Area of Science:

  • Cytogenetics
  • Toxicology
  • Molecular Biology

Background:

  • Thiabendazole (TBZ) is a widely used antiparasitic and food preservative.
  • Understanding its mutagenic and aneugenic effects is crucial for public health.
  • Cytogenetic endpoints provide valuable insights into genotoxicity.

Purpose of the Study:

  • To investigate the mutagenic and aneugenic potential of thiabendazole (TBZ).
  • To characterize the induction of sister chromatid exchanges (SCEs) and mitotic spindle anomalies.
  • To assess TBZ's effects in both Chinese hamster ovary (CHO) cells and human peripheral blood lymphocytes.

Main Methods:

  • Exposure of CHO cells and human lymphocytes to varying concentrations of TBZ.
  • Analysis of sister chromatid exchange (SCE) frequency.
  • Assessment of mitotic spindle anomalies and cell proliferation kinetics (CPK).
  • Evaluation of mitotic index (MI) and replication index (RI) in human lymphocytes.

Main Results:

  • A dose-dependent increase in SCE frequency was observed in CHO cells, particularly with S9-Mix.
  • Significant induction of mitotic spindle anomalies was noted in CHO-K1 cells at higher TBZ concentrations.
  • Human lymphocyte cultures showed a decreased mitotic index (MI) and altered replication index (RI) upon TBZ exposure.
  • Cell proliferation kinetics (CPK) remained unaffected in CHO cells.

Conclusions:

  • Thiabendazole (TBZ) exhibits mutagenic and aneugenic properties, evidenced by increased SCEs and spindle anomalies in vitro.
  • TBZ can disrupt cell division processes in human lymphocytes.
  • The findings highlight the need for careful consideration of TBZ's genotoxic potential in its applications.

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