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Updated: Aug 11, 2026
In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
Published on: October 19, 2018
Intestinal permeability in rats with CCl4-induced portal hypertension
Guo-Xiang Yao1, Zhong-Yi Shen, Xin-Bo Xue
1Department of Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. gxyao@tjh.tjmu.edu.cn
Aim:
To investigate the intestinal barrier changes in rats with CCl4-induced portal hypertension.
Methods:
The permeability of intestinal barrier detected by Lanthanum as a tracer was evaluated in rats. Bacterial translocation and plasma endotoxin were also determined.
Results:
The incidence of bacterial translocation was 85% in rats with CCl4-induced portal hypertension, which was significantly higher than that in control rats (20%, P<0.01). Plasma endotoxin level was significantly higher in experimental group than in control group. Permeability of the epithelial mucosa and pathological alteration were increased in the ileum and the microvilli became shorter and thinner in rats with portal hypertension.
Conclusion:
Bacterial translocation occurs in rats with CCl4-induced portal hypertension and increased permeability between epithelial cells contributes to the translocation.
