Related Experiment Video
Updated: Aug 11, 2026
A Large Animal Model for Acute Kidney Injury by Temporary Bilateral Renal Artery Occlusion
Published on: February 2, 2021
The effect of gradually increased blood flow on ischemia-reperfusion injury in rat kidney
Noreen K Durrani1, Reha Yavuzer, Vijay Mittal
1Department of Surgical Services, Providence Hospital and Medical Centers, 16001 West Nine Mile Road, Southfield, MI 48075, USA.
Background:
Gradually increased blood flow to the ischemic rat kidney was studied to assess the ability to diminish ischemia-reperfusion injury.
Methods:
The left renal artery and vein were isolated in 25 rats. Microclamps were applied for 45 minutes and were released at once (group II) or gradually (group III). Renal arterial blood flow and K+ activity were measured. Bilateral kidneys were harvested for histopathology and for malonyldealdehyde and myeloperoxidase levels.
Results:
Increased K+ activity returned to preischemic values faster in group III than in group II. No statistically significant difference existed in malonyldealdehyde and myeloperoxidase levels; histopathologic scoring showed less tissue damage in group III (P < .05). Contralateral kidney samples showed signs of ischemia in group II.
Conclusions:
Gradually increased blood flow to the ischemic kidney decreases ischemic changes. Ischemic insult to 1 kidney causes histopathologically detectable changes to the contralateral kidney, which can be diminished by gradual reperfusion.
