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Effects of a collagenolytic cell wall component from Fusobacterium necrophorum subsp. necrophorum on rabbit
Keisuke Okamoto1, Masamithu Kanoe, Yukio Yaguchi
1Laboratory of Animal Health, Tokyo University of Agriculture, 1737 Funako, Atsugishi, Kanagawa 243-0034, Japan. heath@qf6.so-net.ne.jp
Abstract:
The effects on rabbit tissue-cultured cells of collagenolytic cell wall component (CCWC) from Fusobacterium necrophorum subsp. necrophorum were investigated. Scanning electron microscopy demonstrated that CCWC damaged the cell surfaces of the rabbit granulocytes and hepatocytes but the effects of the cells differed from each other. Granulocytes appeared smooth and morphologically irregular whereas hepatocytes looked rough and had tiny holes in the cell membranes. Differences in cell viability were observed in MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium, inner salt) assay. The findings suggest that cytotoxic activity in vivo may well contribute to the establishment of an initial injury in visceral tissues, and the action of CCWC could increase the chances of survival for an invading F. necrophorum subsp. necrophorum at the first stages of infection.
