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A study of the effects of pinealectomy on intestinal cell proliferation in infant newborn rats
Marcelo Belini Dalio1, Luis Fernando Haikel Júnior, Renato Belini Dalio
1University Hospital, FMRP, USP, São Paulo, Brazil.
Purpose:
Study the proliferation rate of jejunum and large intestine crypt epithelial cells, in rats pinealectomized immediately after birth.
Methods:
Twenty-four male Wistar rats were distributed into two groups: Acute group (n=12) and Chronic group (n=12). Six animals of each group were operated for removal of the pineal gland (pinealectomy-PnX), and other six were controls (sham pinealectomy-C). Animals from acute and chronic group were sacrificed 15 and 90 days after the surgery, respectively.
Results:
In acute group, pinealectomy of new-born rats has not caused significant alteration in cell proliferation (PnX=58.77+/-1.77 and C=60.88+/-1.10 in the descending colon/PnX=31.56+/-0.45 and C=31.73+/-0.47 in the proximal jejunum) and in crypt cell population (PnX=24.92+/-4.82 and C=23.60+/-2.48 in the descending colon/PnX=39.92+/-3.49 and C=44.32+/-5.56 in the proximal jejunum). However, in chronic group there was an uprising crypt cell production per crypt in the proximal jejunum (PnX=57.54+/-2.19 and C=47.19+/-7.3)and in the descending colon (PnX=37.78+/-2.22 and C=17.92+/-2.28).
Conclusion:
As the increase of intestinal crypts epithelial cells in chronic group is a carcinogenesis predetermining factor, the understanding of the interaction between pineal gland and this event has great importance.
