Isolation of DNA from tumor samples
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Updated: Aug 11, 2026
03:49
Optimized Nuclei Isolation from Fresh and Frozen Solid Tumor Specimens for Multiome Sequencing
Published on: October 13, 2023
Naomi Robertson1, Russell Leek
1Cancer Research UK Molecular Oncology Laboratories, Weatherall Institute of Molecular Medicine, The John Radcliffe Hospital, Oxford, UK.
Methods in Molecular Medicine
|February 24, 2006
Abstract:
A method for producing high-molecular-weight DNA from pulverized tissue, nuclear fractions, or cultured cells. This isolation method relies on the powerful proteolytic activity of proteinase K combined with the denaturing ability of the ionic detergent sodium dodecyl sulfate. Ethylenediaminetetraacetic acid is included in the lysis buffer to inhibit DNases.
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