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Stimulated Single Fiber Electromyography (SFEMG) for Assessing Neuromuscular Junction Transmission in Rodent Models04:30

Stimulated Single Fiber Electromyography (SFEMG) for Assessing Neuromuscular Junction Transmission in Rodent Models

In this study, we demonstrate a refined single fiber electromyography (SFEMG) protocol to allow in vivo measurement of neuromuscular junction (NMJ) transmission in rodent models. A step-by-step approach to the SFEMG technique is described to allow quantification of NMJ transmission variability and failure in rat gastrocnemius muscle.
1.6K
Optimizing Sample Preparation Process for Transmission Electron Microscopy of Neuromuscular Junctions in Drosophila Larvae05:54

Optimizing Sample Preparation Process for Transmission Electron Microscopy of Neuromuscular Junctions in Drosophila Larvae

This report provides a new sample preparation procedure for visualizing neuromuscular junctions in Drosophila Larvae. This method is more effective in preventing the curling of the samples compared to the traditional method and is particularly useful for Drosophila neuromuscular junction ultrastructural...
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Measurement of the Hand Transmitted Vibration of the Human Hand Arm System During Operation of a Hand Tractor09:35

Measurement of the Hand Transmitted Vibration of the Human Hand Arm System During Operation of a Hand Tractor

Here, we present a standardized method for measurement of the hand transmitted vibration from handles of a single-axle tractor with special reference to changes in grip force and vibration...
4.8K
Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions10:45

Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions

The protocol described in this paper uses the mouse levator auris longus (LAL) muscle to record spontaneous and nerve-evoked postsynaptic potentials (current-clamp) and currents (voltage-clamp) at the neuromuscular junction. Use of this technique can provide key insights into mechanisms of synaptic transmission under normal and disease...
9.8K
Hand-held Clinical Photoacoustic Imaging System for Real-time Non-invasive Small Animal Imaging09:43

Hand-held Clinical Photoacoustic Imaging System for Real-time Non-invasive Small Animal Imaging

A clinical handheld photoacoustic imaging system will be demonstrated for real-time non-invasive small animal...
12.1K
Measuring Neuromuscular Junction Functionality10:40

Measuring Neuromuscular Junction Functionality

A functional assessment of the neuromuscular junction (NMJ) can provide essential information on the communication between muscle and nerve. Here we describe a protocol to comprehensively evaluate both the NMJ and muscle functionality using two different muscle-nerve preparations, i.e. soleus-sciatic and diaphragm-phrenic.
18.6K