Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A +TIP for a smooth trip.

Xin Xiang1

  • 1Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA. xxiang@usuhs.mil

The Journal of Cell Biology
|February 24, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Excess p50 induces Arp1-dependent dynactin clusters containing the assembly factor VezA.

bioRxiv : the preprint server for biology·2026
Same author

Comparative Effectiveness of Preoperative Prehabilitation Modalities for Postoperative Recovery in Colorectal Cancer: A Systematic Review and Network Meta-analysis.

Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract·2026
Same author

Integrated proteomic and single-nucleus transcriptomic profiling identifies prognostic markers and therapeutic targets in glioblastoma.

Discover oncology·2026
Same author

Pioneering leadership competencies for evidence-based nursing in China: a groundbreaking application of effective leadership theory.

BMC nursing·2026
Same author

Addressing the critical palliative care gaps in ethnic minority regions of low- and middle-income countries: a focus group study.

BMC medicine·2026
Same author

Effect of transcranial direct current stimulation on perioperative neurocognitive disorder in elderly patients undergoing abdominal tumour surgery: A randomised controlled trial.

European journal of anaesthesiology·2026
Same journal

A pan-vertebrate signaling motif controls the molecular function of intracellular AQP12.

The Journal of cell biology·2026
Same journal

Synergistic assembly, disassembly, and protection of complex forms of bundled F-actin.

The Journal of cell biology·2026
Same journal

Recruitment and release of XPG during NER is controlled by pre- and post-incision factors and EXO1.

The Journal of cell biology·2026
Same journal

Meiotic CENP-C supports centromere assembly and kinetochore recruitment in spermatogenesis.

The Journal of cell biology·2026
Same journal

Phosphatidylserine and RhoB connect PI4P and PA metabolism to maintain plasma membrane identity.

The Journal of cell biology·2026
Same journal

PIKfyve influences inter-organelle contacts with lysosomes to modulate the endoplasmic reticulum.

The Journal of cell biology·2026
See all related articles

A study in budding yeast suggests that the protein Stu2, a microtubule plus-end tracking protein (+TIP), may prevent depolymerizing microtubule tracks from slipping. Stu2 might promote microtubule rescue, stabilizing tracks during chromosome transport.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biophysics

Background:

  • Microtubules are dynamic polymers essential for intracellular transport.
  • Depolymerizing microtubules can pose a challenge for cargo transport, risking detachment.
  • Microtubule plus-end tracking proteins (+TIPs) are known to associate with growing microtubule ends.

Purpose of the Study:

  • To investigate the cellular mechanisms that prevent depolymerizing microtubule tracks from detaching from their cargo.
  • To explore the role of microtubule plus-end tracking proteins (+TIPs) in maintaining microtubule track stability during cargo transport.

Main Methods:

  • Utilized budding yeast as a model organism.
  • Observed chromosome transport dynamics along spindle microtubules.

Related Experiment Videos

  • Investigated the localization and function of the +TIP Stu2 in relation to microtubule dynamics.

    • Main Results:

    • In budding yeast, Stu2, a kinetochore-bound +TIP, was observed to move to the plus end of depolymerizing microtubules during chromosome transport.
    • This movement of Stu2 potentially promotes microtubule rescue, switching depolymerizing ends to polymerizing ones.
    • This mechanism may prevent the microtubule track from "slipping out from under" the chromosome cargo.

    Conclusions:

    • Stu2 plays a crucial role in maintaining microtubule track integrity during chromosome segregation in budding yeast.
    • The findings suggest a novel mechanism where +TIPs actively stabilize depolymerizing microtubule ends to sustain cargo attachment.
    • Further research is warranted to explore if other +TIPs employ similar strategies to stabilize microtubule tracks during vesicular transport.