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Updated: Aug 11, 2026

Confocal Microscopy Analysis of Protein Sorting to Plasmodesmata in Nicotiana benthamiana
Published on: November 1, 2024
Teaching resources. Movement of macromolecules in plant cells through plasmodesmata
Richard A Jorgensen1, William J Lucas
1Department of Plant Sciences, Interdisciplinary Program in Genetics, University of Arizona, Tucson, AZ 85721-0036, USA. raj@ag.arizona.edu
Abstract:
Plasmodesmata are intercellular organelles in plants that allow the passage of molecules between plant cells. Movement through plasmodesmata may allow transcription factors expressed in one cell to move into adjacent cells, thereby regulating gene expression non-cell autonomously. The two animations illustrate (i) movement of a protein through an individual plasmodesma and (ii) an experiment to detect the movement of the transcription factor through plasmodesmata from the L1 layer of a plant meristem into the L2 and L3 layers. These two animations would be useful in teaching plant biology or plant development or a cell biology class discussing mechanisms of intercellular transport.
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