Related Experiment Video
Updated: Aug 19, 2026

Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer
Published on: March 12, 2018
Electrochemical regeneration of nicotinamide adenine dinucleotide
Abstract:
Reduced nicotinamide adenine dinucleotide (NADH) has been characterized electrochemically by solid electrode voltammetry and controlled potential electrolysis. Photometric and enzymatic assay showed that enzymatically active nicotinamide adenine dinucleotide (NAD-+) could be regenerated electrolytically from its reduced form without the use of so-called electron mediators. Complete regeneration of enzymatically active NAD can be expected in pyrophosphate buffers and phosphate buffers during the electrolysis. Advantages of electrochemical regeneration of coenzymes are discussed, especially with regard to immobilization of enzymes.
Related Concept Videos
Electron Transport Chains
The ETC is comprised of...
Electron Carriers
Over the many stages of cellular respiration, glucose breaks down into carbon dioxide and water. Electron carriers pick up electrons lost by glucose in these reactions, temporarily storing and releasing them into the electron...
Batteries and Fuel Cells
Role of Reduced Coenzymes NADH and FADH₂
Oxidation and Reduction of Organic Molecules
The removal of an electron from a molecule, results in a...
Redox Reactions

