[Experimental study of the effects of puerarin on biological characters of osteoblasts in vitro]
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Hong-Min Zang1, Jun-Chang Chen, Yi-Heng Liu
1Department of Orthopedics, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. zhm197@163.com
Objective:
To observe the effect of puerarin on cell proliferation, differentiation, maturation and mineralization in cultured rat osteoblasts.
Method:
Osteoblasts from craniums of newly born SD rats were cultured in vitro. MTT, PNPP and ARS were used to observe the proliferation, activity of ALP and the number of mineral node of cultured osteoblasts in vitro.
Result:
It was found that puerarin had the effect on stimulating cell proliferation, activity of ALP and the number of mineral node of cultured osteoblasts (P < 0.01 or P < 0.05).
Conclusion:
Puerarin can promote proliferation, differentiation, maturation and mineralization of the osteoblasts in vitro.