Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequencing and staphylococci identification.

Alexander Mellmann1, Karsten Becker, Christof von Eiff

  • 1University Hospital Münster, Münster, Germany.

Emerging Infectious Diseases
|February 24, 2006
PubMed
Summary

A new database using the RNA polymerase B (rpoB) gene accurately identifies staphylococcal species and subspecies from clinical samples. This tool aids in the precise identification of staphylococcal infections.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

stackPredAMR-a stacked random forest approach improves AMR phenotype prediction for multiple species and antimicrobial agents.

Bioinformatics advances·2026
Same author

Long-term sequelae after tick-borne encephalitis in Germany: a nationwide claims-based matched cohort study.

Ticks and tick-borne diseases·2026
Same author

Factors Governing the Cross-Species Virulence of Shiga Toxin-Producing <i>Escherichia coli</i>.

Pathogens (Basel, Switzerland)·2026
Same author

Cost-Effectiveness of Alternative Immunization Strategies to Protect Infants Against Respiratory Syncytial Virus in Germany: A Decision Modeling Study.

Infectious diseases and therapy·2026
Same author

A Comparison of Endodontic Microbiomes Associated With Symptomatic and Asymptomatic Apical Periodontitis by Next-Generation Sequencing.

International endodontic journal·2026
Same author

Chlorine dioxide flushing protocols for microbial reduction in dental chair units.

PloS one·2026

Area of Science:

  • Microbiology
  • Genetics
  • Bioinformatics

Background:

  • Staphylococcal infections pose a growing clinical challenge.
  • Accurate identification of staphylococcal species and subspecies is crucial for effective treatment.
  • Existing identification methods may require improvement for clinical settings.

Purpose of the Study:

  • To create a comprehensive reference database of partial RNA polymerase B (rpoB) gene sequences.
  • To evaluate the accuracy of this database for identifying clinical staphylococcal isolates.
  • To improve the diagnostic capabilities for staphylococcal infections.

Main Methods:

  • Sequencing of the partial rpoB gene (nucleotides 1444-1928) from type strains of all staphylococcal species and subspecies.
  • Development of a reference database using these sequences.

Related Experiment Videos

  • Testing the database against 55 clinical staphylococcal isolates.

    • Main Results:

    • The rpoB gene database successfully identified all 55 clinical staphylococcal isolates at the species level.
    • The database demonstrated high accuracy at the subspecies level, with only 2 misidentifications.
    • This indicates the robustness of the rpoB gene for staphylococcal identification.

    Conclusions:

    • A reference database of partial rpoB gene sequences is a reliable tool for identifying staphylococcal species.
    • The established database shows high accuracy for subspecies identification, aiding clinical diagnostics.
    • This approach offers a valuable method for the precise identification of staphylococcal pathogens.