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rev-like transcripts of caprine arthritis encephalitis virus
H Kalinski1, A Yaniv, P Mashiah
1Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Israel.
Virology
|August 1, 1991
Summary
Caprine arthritis encephalitis virus (CAEV) gene expression in tahr lung cells is complex, involving temporally regulated, alternatively spliced transcripts. These transcripts can potentially encode novel viral proteins, including a Rev-like protein.
Area of Science:
- Virology
- Molecular Biology
- Genomics
Background:
- Caprine arthritis encephalitis virus (CAEV) is a lentivirus known to cause disease in goats.
- Understanding the complex gene expression patterns of CAEV is crucial for developing effective control strategies.
Purpose of the Study:
- To investigate the pattern of CAEV genome expression in acutely infected tahr lung cells.
- To elucidate the genetic structure and potential protein products of CAEV transcripts.
Main Methods:
- Northern blot analysis to detect CAEV-specific transcripts.
- Nucleotide sequence analysis of isolated cDNA from infected tahr cells.
- Analysis of exon-intron structure and potential protein-coding regions.
Main Results:
- Five CAEV-specific transcripts (9, 6.5, 5.0, 2.5, and 1.4 kb) were detected.
- Two cDNA species representing rev-like transcripts were identified, with distinct exon compositions.
- These transcripts could potentially encode a Rev-like protein, a truncated Env protein, and a novel protein (X).
Conclusions:
- CAEV exhibits a complex gene expression strategy, similar to other lentiviruses.
- Alternative splicing and the production of potentially polycistronic transcripts are key features of CAEV gene expression.
- The identified transcripts and potential proteins offer insights into CAEV replication and pathogenesis.