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Defective interfering particles of human parainfluenza virus type 3 are associated with persistent infection in cell

A Moscona1

  • 1Department of Pediatrics, Mount Sinai School of Medicine, New York, New York 10029-6574.

Virology
|August 1, 1991
PubMed

Insights

Defective-interfering (DI) particles containing subgenomic RNAs were found in persistent human parainfluenza virus type 3 (HPF3) infections. These DI particles interfere with wild-type virus growth, suggesting a role in HPF3 persistence.

Area of Science:

  • Virology
  • Molecular Biology
  • Cell Biology

Background:

  • Persistent infections with human parainfluenza virus type 3 (HPF3) are characterized by the presence of viral RNA.
  • Subgenomic RNAs have been observed in CV-1 cell lines persistently infected with HPF3.

Purpose of the Study:

  • To identify the nature of subgenomic RNAs in persistently infected cells.
  • To investigate the role of these RNAs in viral interference and persistence.

Main Methods:

  • Ultracentrifugation to isolate viral particles.
  • Analysis of RNA content in infected cell lines.
  • Interference assays with wild-type virus.

Main Results:

  • Distinct subgenomic RNAs, identified as defective-interfering (DI) particle genomes, were found in persistently infected CV-1 cells.
  • DI particles were present in the culture fluid and interfered with wild-type HPF3 growth.
  • Removal of DI particles eliminated inhibitory activity, indicating the effect is particle-mediated, not soluble factors.

Conclusions:

  • Defective-interfering particles play a significant role in the biology of persistent HPF3 infections.
  • DI particles may contribute to the maintenance of viral persistence by interfering with wild-type virus replication.

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